《植物生理学报》 2017, 53(11): 2007-2012

苹果抗寒半矮化砧木‘54-118’的组织培养及其离体叶片不定梢再生

孙清荣^*, 关秋竹, 孙洪雁, 李林光, 陶吉寒, 王海波, 何平

山东省果树研究所, 山东泰安27100

通信作者：孙清荣；E-mail: sdipss@163.com

摘　要：

本文以苹果抗寒半矮化砧木‘54-118’为试材, 研究基本培养基及植物生长调节物质对无菌苗增殖和生根的影响; 并以无菌苗离体叶片为外植体, 研究基本培养基、植物生长调节物质及碳源对叶片不定梢再生的影响。结果表明, 无菌苗的适宜增殖培养基为QL+1 mg·L^-1 6-BA+0.3 mg·L^-1 IBA+30 g·L^-1蔗糖, 增殖系数为5.1。适宜的生根培养基为1/4MS+0.5 mg·L^-1 IBA+20 g·L^-1蔗糖, 生根率为77.9%, 平均每株根数为3.5。离体叶片不定梢再生的最佳培养基为改良MS (NM)+3 mg·L^-1 6-BA+0.3 mg·L^-1 IBA+30 g·L^-1 D-山梨醇, 再生率为85.6%。

关键词：苹果砧木; 增殖; 生根; 离体叶片; 不定梢再生

收稿：2017-06-15   修定：2017-10-21

资助：山东省农业良种工程项目(鲁科字[2014]96)和山东省重点研发计划项目(2016GNC113008)。

Tissue culture and shoot regeneration from leaf explants of cold-hardy and semi-dwarf apple rootstock ‘54-118’

SUN Qing-Rong^*, GUAN Qiu-Zhu, SUN Hong-Yan, LI Lin-Guang, TAO Ji-Han, WANG Hai-Bo, HE Ping

Shandong Institute of Pomology, Taian, Shandong 271000, China

Corresponding author: SUN Qing-Rong; E-mail: sdipss@163.com

Abstract:

Cold-hardy and semi-dwarf apple rootstock ‘54-118’ was selected as material, the effects of basal medium composition and plant growth regulators on proliferation and rooting of in vitro shoots were examined. In vitro leaves were selected as explants, the effects of basal medium composition, plant growth regulators and carbon sources on shoot regeneration were investigated. The results showed that the optimal proliferation medium was QL+1 mg·L^-1 6-BA+0.3 mg·L^-1 IBA+30 g·L^-1 sucrose, multiplication rate was 5.1. The optimal rooting medium was 1/4MS+0.5 mg·L^-1 IBA+20 g·L^-1 sucrose, rooting rate was 77.9%, average root number was 3.5. The optimal shoot regeneration medium was modified MS (NM)+3 mg·L^-1 6-BA+0.3 mg·L^-1 IBA+30 g·L^-1 D-sorbitol, regeneration frequency reached 85.6%.

Key words: apple rootstock; proliferation; in vitro rooting; in vitro leaves; shoot regeneration